인체편평세포암세포주에서 9-HpbD-a과 632nm 다이오드 레이저를 이용한 광역학치료의 항암효과
Anticancer effect of photodynamic therapy using 9HpbD-a and 632nm diode laser on human squamous cell carcinoma
오충훈, 천재식, 정필상, 안진철,
소속 상세정보
오충훈 ( Oh Chung-Hun ) - 단국대학교 치과대학
천재식 ( Chun Gae-Sig ) - 단국대학교 치과대학 구강생리학교실
정필상 ( Jung Pil-Sang ) - 단국대학교 의과대학 병리학교실
안진철 ( Ahn Jin-Chul ) - 단국대학교 의과대학 의학레이저연구센터
KMID : 0829220050290020075
Abstract
This study was conducted to test the anticancer effect of photodynamic therapy using chlorophyll derivative (9-HpbD-a) and 632nm diode laser. Human SNU 1041 cells were seeded into 96 well plate of 104cells/well and cultured for 24 hours. Cells were washed with media containing various concentration of 9-HpbD-a ranging from 0ug/ml to 3.75ug/ml. Then 632 nm diode laser was given at various lasering time setting, and at various starting time after initial 24 hours of culture. The treated cells were incubated 48 hours and tetrazolium-based colorimetric(MTT) assay was done to measure the viability of cells. For in vivo study, SNU-1041 cells were xenografted into the back of nude mouse. When the xenografted tumors grew up to 400-600 mm3, the animals were randomly placed into 4 groups: Group I (n=20), PDT group, interstitial injection of 9-HpbD-a (47 ug/kg) followed by irradiation with 3.2 J/㎠ of light 6 hours after then injection; Group II (n=10), irradiation with 3.2 J/㎠ of light using diode laser; Group III (n=10), interstitial injection of 9-HpbD-a only(47 ug/kg); Group IV (n=10), normal control group. The viability of cells was decreased with increasing lasering time. No significant difference of cell viability was noted by variously delayed starting time of lasering. PDT effects were observed in the xenografted nude mouse model. Group IV (no 9-HpbD-a, no laser irradiation) was a control group which showed a continuous tumor growth. Group III (9-HpbD-a injection only) showed no response, Group II (laser irradiation only) showed 1 complete remission out of 10 (10%), Group I (9-HpbD-a and laser irradiation) showed 13 cpmplete remission out of 20 (65%), Group I showed significant remission rate, comparing to other groups (p<0.05). This study demonstrated anticancer effect of photodynamic therapy using 9-HpbD-a and 632nm diode laser on human squamous cell carcinoma cell line.
키워드
Photodynamic therapy;9-hydroxypheophorbide-a;632 nm diode laser
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